The three Spider-Man movies alone have generated some $2.5 billion in worldwide box office receipts.
The acquisition reflects Disney's long-term strategy of adding to its cast of characters and talent as when it bought animation powerhouse Pixar for $7.4 billion in 2006.
The Marvel deal also gives Disney a key brand for connecting with teenage boys, a market that has been overshadowed by the popularity of stars such as Hannah Montana with girls.
'Disney is the perfect home for Marvel's fantastic library of characters given its proven ability to expand content creation and licensing businesses,' said Marvel chief executive Ike Perlmutter.
'This is an unparalleled opportunity for Marvel to build upon its vibrant brand and character properties by accessing Disney's tremendous global organization and infrastructure around the world,' he added.