Traffic on the overland route was restored early Monday morning.
The highway running through Chaman is one of the two main supply routes used by contractors to ferry fuel and military supplies from Pakistan's southern port city of Karachi to the US and NATO troops in landlocked Afghanistan.
Militants have staged relatively fewer attacks on the Chaman road link than on the main Khyber Pass route in Pakistan's north-western tribal region.
Pakistani authorities have increased checking of lorries coming from Afghanistan since April 4 after around 50 Afghans, who were being smuggled to Iran via Balochistan, were found dead in a container truck left abandoned in the provincial capital, Quetta.