The fine is equal to two percent of AmBev's turnover in 2003, the year before the Cade investigation began.
AmBev merged in 2004 with Belgium's Interbrew and the new conglomerate, InBev, last year acquired US titan Anheuser-Busch to create Anheuser-Busch InBev, the world's largest brewer.
Besides imposing the fine, Cade ordered AmBev to discontinue retailer loyalty programs that include an exclusivity clause.
The investigation was spurred by a complaint from Brazilian brewer Schincariol, which claimed that AmBev's practices caused sales of the smaller firm's Nova Schin and Kaiser to fall 20 percent.